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KMID : 1005220100250040221
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Journal of Embryo Transfer
2010 Volume.25 No. 4 p.221 ~ p.227
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Selection of Early Cleaved Embryos and Optimal Recipients to Improve Efficiency of Pig Cloning
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Koo Ok-Jae
Lee Dong-Won
Kang Jung-Taek
Kwon Dae-Kee
Park Jung-Hee
Park Sol-Ji
Kim Su-Jin
Jang Goo
Lee Byeong-Chun
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Abstract
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Early cleavage is a reliable prognostic tool for successful embryo transfer in assisted reproduction because early cleaved embryo show better pregnancy rate after transfer. There for, preparation of good embryo recipient is important factor to optimize efficiency of pig cloning. The present study was performed to evaluate the effect of early cleavage on the in vivo development of cloned embryos and to analyze breed, parity and estrous synchrony to optimize recipient for pig cloning. In vitro matured porcine oocytes derived from local slaughterhouse and fibroblasts derived from miniature pig fetuses were used for somatic cell nuclear transfer (SCNT). Reconstructed embryos were transferred to recipient pigs on the same day of SCNT or after 1~2 days of in vitro culture for selecting early cleaved embryos. Breed, parity and date of standing estrous of recipients were recorded for analysis. After 25~35 days after embryo transfer pregnancy was diagnosed using ultrasonography, and pregnant recipients were monitored till delivery. Between purebred and crossbred, no significant difference was founded in both pregnancy and delivery rates. However, early cleaved embryos showed significantly higher pregnancy (46.2%) and delivery (12.8%) rates compared to non-selectively transferred group (24.8% and 4.5%, respectively). The results also showed that the recipients showing standing estrous on the same day of SCNT and less than 4 parities were most suitable for pig cloning.
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KEYWORD
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breed, early cleavage, parity, pig cloning, recipient
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